Next-generation sequencing reads are trimmed of low-quality bases, and short or otherwise inferior reads are discarded.
Examap, a novel, codon-aware reference mapping tool, uses an assembly-free approach to map sequence reads to one or more reference sequences while considering both sequencing error and true diversity.
Mutations are called in each individual read. The Examap algorithm allows mutations to be called with >99% per-read accuracy.
Relevant genes and mutations, at various prevalence levels, are reported and, if relevant, analysed using gold-standard drug-resistance testing algorithms.
The Exatype NGS SARS-CoV-2 solution supports the Illumina and Ion Torrent sequencing platforms.
The Exatype NGS HIV solution supports the Illumina and Ion Torrent sequencing platforms.
The Exatype Sanger HIV, solution supports any sequencing by capillary electrophoresis (CE), e.g. the Applied Biosystems 3730 and 3500 Series Genetic Analyzers.